Olive text indicates a specimen that
has not been thoroughly examined (for example, for
microscopic details) and marks other places in the text
where data is missing or uncertain.
The following material is derived from photographs and
notes of the collector, molecular research of Dr. L. V.
Kudzma and other original research of R. E. Tulloss.
lamella edge tissue
Ontario, Canada: In mixed woods with
Acer saccharum, and Betula
Florida, U.S.A.: In office park landscaping
Quercus and Lagerstroemia.
Texas, U.S.A.: In sandy soil of forest including
Quercus and Pinus.
ONTARIO—Port Cover, lynn Valley Rd. &
Haldimand Rd. 70 [42.8380° N/ 80.1211° W, 201 m],
30 viii.2018 Eva Skivic s.n.
(RET 841-5, nrITS-LSU seq'd.).
Roseneath, Peter's Woods
Prov. Nat. Reserve, 15.vii.2014 Jan Thornhill s.n.
(RET 637-3, nrITS & nrLSU seq'd.).
FLORIDA—Leon Co. - Tallahassee
[30.4264º N/ 84.2847º W, 62 m],15.vi.2017 Russell
de Grove 2-092106 (RET 782-8, nrITS-LSU seq'd.),
22.vi.2017 R. de Grove s.n. [mushroomobserver
(RET 783-7, nrITS-LSU seq'd.); unkn. loc., 15.vi.2017
Russell de Grove 3-151449-092128 (RET 782-4, nrITS-LSU
seq'd.); unkn. loc., 15.vi.2017 Russell de Grove
4-145103 (RET 782-9), nrITS-LSU seq'd.).
Co. - Big Thicket Nat. Preserve - North Turkey Creek
Unit [30°36.164’ N/ 94°20.913’ W, 45.1 m], 11.vi.2015 Ronald L.
Pastorino 6-11-14K [mushroomobserver
(RET 629-2, nrITS & nrLSU seq'd.).
WISCONSIN—Walworth Co., Lulu Lake
St. Natural Area [42.8362° N/ 88.4525° W, 252 m],
2.ix.2018 Andrew Khitsun s.n. [mushroomobserver
(RET 850-7, nrITS-LSU seq'd.).
A comparison of the nrITS gene from four collections
(RET 629-2, RET 637-3, RET 850-7, and Mycomap 9697)
was made by alignment of the sequences.
In addition to the
sequence segment posted by Stephen, Karen Hughes
(Univ. Tenn., Knoxville) and Linas Kudzma also produced
relevant sequences. If we look at all the data,
we have data extending from within the ITS1F primer
site in nrSSU into nrLSU. The overall aligned
region is 746 characters long.
The 5' terminus of nrITS falls at character position
The 3' terminus of nrITS falls at character
Mycomap 9697 runs between character
positions 29 and 661 and
includes the entire nrITS.
A rough characterization of the sequences based on
the alignment follows:
char. 143 is a G in the RET 629-2 data, but
an A in the other sets of data.
At around char. 248-250 there is a multi-"A"
repeat. The length differs by up to two
characters. This could occur naturally or in
the process of copying the DNA for sequencing.
It is a difference without any weight to it.
Mycomap 9697 is distinguished by having a "T" at
position 248 prior to the repeat.
char. 277 is a C in RET 629-2, RET 850-7,
and Mycomap 9697 while in
the other data there is a
clear T (the raw data sometimes shows a T combined
C as a distinctly lesser peak). Maybe a SNP?
The same is true for char. 288.
Char. 304 is an A in RET 629-2, RET
850-7, and Mycomap 9697. It is a G in the other
That is the complete list of differences.
Summary: At minimum, there are two 2 invariant
two possible differences (if the SNP suggestions
don't hold up to further examination).
The Ontario material (RET 637-3) was briefly treated
on the present site as "Amanita
sp-pseudocrocea02." The species has been more
treated under the names "A. sp-F20" and
—R. E. Tulloss and Linas Kudzma
Information to support the viewer in reading the content of "technical" tabs
can be found here.
Tulloss, R. L. Pastorino, S. D. Russell & Kudzma
double click in markup mode to edit.
1. Amanita aurorae, Big Thicket Nat. Preserve, Tyler Co., Texas, USA. (RET 629-2)
2. Amanita aurorae, Big Thicket Nat. Preserve, Tyler Co., Texas, USA. (RET 629-2)
3. Amanita aurorae, Lulu Lake St. Natural Area, Walworth County, Wisconsin, USA. (RET 850-7)
4. Amanita aurorae, aging, Tallahassee, Leon County, Florida, USA. (RET 783-7)
5. Amanita aurorae, Tallahassee, Leon County, Florida, USA. (RET 783-6)
Ronald L. Pastorino - (1) Big Thicket National Preserve,
Tyler County, Texas, U.S.A. (RET 629-2)
[Note: Original images can be found here.]
Spore data for collections provisionally identified as: Amanita aurorae Tulloss, R. L. Pastorino, S. D. Russell & Kudzma
Each spore data set is intended to comprise a set of measurements from a single specimen made by a single observer;
and explanations prepared for this site talk about specimen-observer pairs associated with each data set.
Combining more data into a single data set is non-optimal because it obscures observer differences
(which may be valuable for instructional purposes, for example) and may obscure instances in which
a single collection inadvertently contains a mixture of taxa.